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The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatants 48 h later。

HEK293T cells were cotransfected with pCF-BBtr, the empty vector pcDNA, or pcfeA3Z2b-HA, and increasing amounts of plasmids encfefaboding either wt or mutant Bet (Figure。

Fe3 Reduces the Titer of bet-Deficient FFV and Induces Genome Editing. The effect of fe3 coexpression with WT and bet -deficient FFV genomes was studied after transfection of。

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